张玮^1，， 王辉^1，， 李宏博^1，， 杜云霞^1，， 邢玲玲^2，， 徐宁^1，， 刘青娟^1，*

（  1、河北医科大学病理教研室；       2、河北医科大学第二医院肾内科；  ）

摘要： 目的：探讨抑瘤素M（oncostatin M，OSM）在狼疮鼠肾组织中的表达及意义。方法：以MRL/MpJ鼠作为正常对照鼠，MRL/lpr鼠随作为狼疮鼠。收集动物血、尿进行生化指标检测；收集肾组织后免疫组织化学和酶联免疫吸附实验检测OSM的表达情况；Western蛋白印迹法检测E-钙黏蛋白（E-cadherin）、α-平滑肌肌动蛋白（α-smooth muscle actin, α-SMA）的表达。结果：与对照鼠相比，狼疮鼠肾组织中OSM的表达明显增多，且其表达与血尿素氮(r=0.892, p<0.01)、24 h尿蛋白(r=0.828, p<0.01)及α- SMA的表达呈正相关(r=0.882, p<0.01)，而与E-cadherin的表达呈负相关（r=-0.852, p<0.01）。结论：OSM在狼疮鼠肾组织中的表达升高并与狼疮鼠肾功能减退及肾小管上皮细胞转分化有一定的关系。
关键词： 狼疮性肾炎；MRL/lpr小鼠；抑瘤素M；肾小管上皮细胞

ZHANG Wei^1,， WANG Hui^1,， LI Hongbo^1,， DU Yunxia^1,， XING Lingling^2,， XU Ning^1,， LIU Qingjuan^1,*

（  1、Department of Pathology, Hebei Medical University；       2、Department of Nephrology, the Second Hospital of Hebei Medical University；  ）

Abstract： Object: In order to investigate the expression of oncostatin M (OSM) in the renal cortex of MRL/lpr mice. Method: MRL/MpJ mice were designated as the control group and MRL/lpr mice were designated as the lupus group. The expression of OSM was detected by the methods of immunohistochemistry and enzyme linked immunosorbent assay. Western blotting analysis was used to determine the expression of E-cadherin and α-SMA. Results: Compared with control mice, the OSM expression increased in lupus mice. And the expression level of OSM is positively correlated with blood urea nitrogen (r=0.892, p<0.01), 24 h urinary protein (r=0.828, p<0.01) and the expression level of α-SMA (r=0.882, p<0.01) but is negatively correlated with the expression level of E-cadherin (r=-0.852, p<0.01). Conclusion: The OSM expression is increased in kidney in lupus mice, which maybe connected with the renal dysfunction and tubular-epithelial cell transdifferentiation.
Keywords： lupus nephritis; MRL/lpr mice; oncostatin M; tubular epithelial cell

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