欧阳慧子^1，， 刘华明^2，， 何俊^2，*

（  1、天津中医药大学第一附属医院，天津，300193；       2、天津市现代中药重点实验室, 天津中医药大学, 天津，300193；  ）

摘要： [目的] 考察杠柳苷元经大鼠肝微粒体体外代谢的特点，对其代谢产物进行定性研究。[方法] 建立肝微粒体温孵体系，采用超高效液相串联飞行时间质谱技术，结合保留时间、精确质量数和离子的碎片特征对孵育后的代谢产物进行结构鉴定。[结果] 杠柳苷元经肝微粒体温孵后，可以检测到除原型以外的4个I相代谢产物。[结论] 杠柳苷元经大鼠肝微粒体温孵后，主要的代谢途径为脱水、脱氢和甲基化，为其体内代谢的研究提供了基础。
关键词： 代谢；杠柳苷元；大鼠肝微粒体；液相色谱-高分辨率质谱

Ouyang Huizi^1,， Liu Huaming^2,， He Jun^2,*

（  1、First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin,300193；      2、Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China；  ）

Abstract： [Objective] The metabolites of periplogenin in rat liver microsomes were studied using UPLC-Q-TOF/MS. [Methods] After establish the rat liver microsomal incubation system, the metabolites of periplogenin in rat liver microsomes were detected by ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combine with retention time, exact mass number and fragmentation characteristics of the ions. [Result] Besides of the parent component, four metabolites were determined as phase I metabolites in the rat liver microsomal incubation. [Conclusion] The results indicated that the dehydration, dehydrogenation and methylation were the major phase I metabolic pathways of periplogenin in vitro. The present study provided the theoretical basis for metabolism in vivo.
Keywords： metabolism; periplogenin; rat liver microsomes; Liquid chromatography-High resolution mass spectrometry

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