孙丽宁^1，， 孙越^1，， 齐军^2，， 王雪芹^3，， 李克忠^1，*

（  1、山东大学第二医院麻醉科；       2、烟台毓璜顶医院麻醉科；       3、临沂市人民医院麻醉科； ）

摘要： 目的 观察吸入不同时间异氟醚对发育期大鼠海马组织β-catenin的蛋白表达的影响。方法 出生后7天雄性SD大鼠32只随机分成4组:吸入空气的对照组（A组），吸入2%异氟醚2小时组（B组），吸入2%异氟醚4小时组(C组)，以及吸入2%异氟醚6小时组（D组），每组幼鼠处理中均无缺氧及CO2蓄积发生。实验结束 6小时后,将上述各组幼鼠随机分为RT-PCR实验组和western blot实验组，分别取海马组织检测β-catenin蛋白表达。结果 发育期SD大鼠海马组织β-catenin mRNA的表达：与对照组相比，B组和D组有统计学差异（P<0.05），C组表达虽有上调，但无统计学意义；发育期SD大鼠海马组织β-catenin蛋白的表达：与对照组比，B组、C组和D组明显上调(P<0.05)， 其中D组显著上调（P<0.01）。结论 吸入2%异氟醚处理会导致发育期大鼠海马组织β-catenin蛋白的表达上调。
关键词： 吸入麻醉；异氟醚；海马组织；β-catenin

SUN Lining^1,， SUN Yue^2,， QI Jun^3,， WANG Xueqin^4,， LI Kezhong^5,*

（  1、Department of Anethesiology, The Second Hospital of Shan Dong University, Jinan 250000 ；       2、Department of Anesthesiology, The Second Hospital of Shan Dong University, Jinan 250000；       3、Department of Anethesiology, Yan Tai Yuhuangding Hospital, Yantai 264000；       4、Department of Anethesiology, Linyi People's Hospital, Linyi 276000 ；      5、Department of Anethesiology, The Second Hospital of Shan Dong University, Jinan 250000；  ）

Abstract： Objective To compare the effect of different time course of isoflurane on β-catenin expression in the hippocampus of developing rats. Method Thirty-two 7-day-old SD male rats were randomly divided into four groups: control group (A) inhalation of O2 instead of isoflurane ,inhalation of 2% isoflurane 2 hours group (B) , inhalation of 2% isoflurane 4 hours group (C) and inhalation of 2% isoflurane 6 hours group (D). Rats in every group were no hypoxia and CO2 accumulation occurred in the process. 6 hours after treatment rats in every group were killed and hippocampus were removed immediately . Results In the group of PCR, compared with control group, the level of β-catenin was increased markedly (P<0.05) only in groups B and D. While group C has raised, there was no statistical significance; In the group of western bolt, compared with control group, the expression of β-catenin in groups of B, C, D were raised markedly (P<0.05), in which the group D rised significantly (P<0.01). Conclusions Treatment with 2% isoflurane can result in upregulation of β-catenin expression in the hippocampus of developing rats.
Keywords： Inhalation Anesthesia; Isoflurane; Hippocampus; β-catenin

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