李喜功^1，*， 陆洋^2，， 杜俊华^1，， 徐三中^1，， 林向进^1，， 凌志恒^1，

（  1、浙江大学附属第一医院,杭州,310058；       2、浙江大学附属第一医院,杭州,310058 ；  ）

摘要： 目的:在大鼠创伤性脊髓损伤模型中研究罗格列酮的神经保护作用。 方法:成年SD大鼠（n=12/组）分为椎板切开术（假手术组），单纯脊髓损伤组，脊髓损伤+罗格列酮治疗组（2mg/kg BID,7天），脊髓损伤+生理盐水组（溶剂对照组）。脊髓损伤通过硬膜外施加动脉夹实现。脊髓损伤后24小时，对脊髓细胞凋亡和肿瘤坏死因子α（TNFα）表达水平，白介素1β（IL-1β），过氧化物酶（MPO），凋亡相关蛋白B细胞淋巴瘤2（Bcl-2）和B细胞淋巴瘤2相关X蛋白（Bax）进行检测。在脊髓损伤后3，7，10，14和21天进行运动功能测试。 结果:脊髓损伤后24小时，罗格列酮组的TNFα，IL-1β和MPO水平和细胞凋亡显著低于单纯脊髓损伤组和溶剂对照组。罗格列酮可以逆转脊髓损伤引起的Bax升高和Bcl-2下降。罗格列酮组的大鼠比单纯脊髓损伤组和溶剂对照组明显更好的功能恢复。 结论:罗格列酮显著提高大鼠脊髓损伤后的功能恢复。这一作用可能是通过减少局部炎症因子的浓度和细胞凋亡的表达实现。
关键词： 细胞生物学；细胞凋亡；炎症因子；神经保护；罗格列酮；脊髓损伤

Li Xigong^*， Lu Yang， Du Junhua， Xu Sanzhong， Lin Xiangjin， Ling Zhiheng

（  Zhejiang University, School of Medicine, First Affiliated Hospital,Hangzhou,310058；  ）

Abstract： Objective: To investigate the neuroprotective effects of rosiglitazone in a rat traumatic spinal cord injury (SCI) model. Methods: Adult Sprague-Dawley rats (n ? 12/group) underwent laminectomy (sham), SCI, SCI and rosiglitazone treatment (2 mg/kg twice daily for 7 days), or SCI and saline injection (vehicle). SCI was induced via dural application of an aneurysm clip. Spinal cord apoptosis and levels of tumour necrosis factor-a (TNFa), interleukin (IL)-1b, myeloperoxidase (MPO) and the apoptosisassociated proteins B-cell leukaemia/lymphoma 2 (Bcl-2) and Bcl-2 associated X protein (Bax) were examined 24 h after SCI. Locomotor function was evaluated 3, 7, 10, 14 and 21 days after SCI. Results: At 24 h after SCI, apoptosis and TNFa, IL-1b and MPO concentrations were significantly lower in the rosiglitazone group than in the vehicle and SCI groups. SCI resulted in an increase in Bax and a decrease in Bcl-2, which was reversed by rosiglitazone treatment. Rats in the rosiglitazone group had significantly better functional recovery than those in the vehicle and SCI groups. Conclusion: Rosiglitazone signiEcantly improved functional recovery, probably via attenuation of the local inflammatory reaction and reduced apoptosis.
Keywords： cytobiology; apoptosis; inflammation; neuroprotection; rosiglitazone; spinal cord injur 

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