DHA通过调控脂肪合成代谢影响大黄鱼肝脂沉积

摘要： 脂肪异常沉积严重损害养殖鱼类健康。本实验从DHA对大黄鱼肝组织细胞脂肪合成代谢的影响入手，离体探究其对脂沉积的影响。结果发现，DHA水平的升高显著降低了大黄鱼肝组织细胞中甘油三酯的含量（P<0.05）。DHA水平的升高显著降低了大黄鱼肝组织细胞脂肪酸合成酶（FAS）基因的表达量（P<0.05），而对葡糖六磷酸脱氢酶（G6PD）基因的表达量没有显著性影响（P>0.05）。转录因子肝X受体α（LXRα）及固醇调节元件结合蛋白1（SREBP-1）在脂肪合成代谢中起着极其重要的作用。结果发现，DHA水平的升高显著抑制了LXRα以及SREBP-1基因的表达量（P<0.05）。综上，DHA可以通过抑制大黄鱼肝细胞脂肪合成来抑制脂肪沉积，该过程可能是通过转录因子LXRα以及SREBP-1来介导的。
关键词： 水产养殖；DHA；脂肪合成；脂肪沉积；大黄鱼

LI Songlin， LIAO Kai， YUAN Yuhui， WANG Tianjiao， XU Wei， MAI Kangsen， AI Qinghui^*

（  Ocean University of China, Fisheries College, Qingdao 266003；  ）

Abstract： Abnormal lipid deposition seriously impacted the health of farmed fish. The present study was started from the influence of DHA on lipogenesis to investigate its regulation on lipid deposition of large yellow croaker in vitro. Results showed that the increasing DHA content significantly down-regulated the TG content in primary hepatocyte of large yellow croaker (P<0.05). DHA significantly repressed the transcription of fatty acid synthesis (FAS)(P<0.05), while no significant impact on G6PD(P>0.05). Some transcription factors, such as liver X receptor α (LXRα) and sterol regulatory element-binding protein-1 (SREBP-1)，played vital important roles in lipid metabolism. DHA significant down-regulated the transcription of LXRα and SREBP-1(P<0.05). In conclusion, DHA could alleviate lipid deposition through inhibiting lipogenesis, which could be mediated via LXRα and SREBP-1.
Keywords： Aquaculture; DHA; lipogenesis; lipid deposition; large yellow croaker

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