miR-30b： 一个肌肉MyoD表达的调控因子职称论文发表

摘要： MyoD是一个调控罗非鱼肌肉发育的关键因子，如何精细调控MyoD表达水平对于肌肉的发育至关重要。研究发现，miRNA可以在转录后水平发挥调控作用，且可以参与肌肉的发育。因此，可以推测miRNA有望通过调控MyoD表达发挥从而影响肌肉的发育过程。 生物信息预测发现，MyoD的3'-UTR区具有miR-203b的结合位点。miR-203b与MyoD表达呈现负相关，而且miR-203b的表达干预可以影响MyoD的下游基因表达。总之，本研究发现了miRNA介导的肌肉发育的调控新机制。
关键词： 水产养殖; 罗非鱼; miR-203b; miRNA；肌肉发育

Yan Biao^1,*， Wang Zhenhua^2,， Zhao Jinliang^1,

（  1、College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306；       2、College of Information Technology, Shanghai Ocean University, Shanghai, 201306；  ）

Abstract： MyoD is one of the helix-loop-helix proteins regulating muscle-specific gene expression in tilapia. Tight regulation of the MyoD protein level is necessary for the precise regulation of skeletal muscle development. MicroRNAs (miRNAs) are a class of regulatory RNAs that post-transcriptionally regulate gene expression. An increasing amount of evidence has suggested that miRNAs play an important role in regulating skeletal muscle development. We reasoned that MyoD expression may be regulated by miRNAs. Predictions from bioinformatics have identified a putative miR-203b target site in the 3′-UTR of the MyoD gene. Interestingly, miR-203b expression is negatively correlated with MyoD expression, whereas miR-203b suppression leads to a significant increase in MyoD expression, thereby activating MyoD downstream genes. A 3′-UTR luciferase reporter assay further verifies the direct interaction between miR-203b and MyoD. Taken together, our results reveal a novel molecular mechanism inwhich miRNA participates in transcriptional circuits that regulate gene expression in tilapia skeletal muscle.?
Keywords： Aquaclture, Nile tilapia, miR-203b, microRNA, skeletal muscle development.