张玲莉^1，， 孙忠广^1，， 雷乐^2，， 沈超^1，， 邹军^3，*

（  1、上海体育学院运动科学学院，上海 200438；       2、上海体育学院经济管理学院，上海 200438；       3、上海体育学院发展规划处，上海 200438；  ）

摘要： 目的：本实验通过给小鼠注射Smad1激活酶抑制剂后加以跑台运动，检测运动训练对小鼠骨密度、骨生物力学性能以及相关血液尿液指标的影响，初步揭示骨形态发生蛋白信号通路在运动训练调节骨代谢中的作用。方法：72只6周龄C57BL/6雄性小鼠分为对照组、运动组、LDN组、运动+LDN组，每组18只，分别予以小鼠中等强度跑台训练和（或）注射LDN-193189HCL（BMP-Smad1 阻滞剂）。干预结束后检测小鼠股骨骨密度、骨生物力学性能（最大载荷、弯曲强度、弹性模量）以及血清中钙、磷浓度和尿液脱氧吡啶啉排泄率等指标。结果：运动组小鼠股骨骨密度显著高于对照组小鼠（P＜0.01），运动+LDN组小鼠股骨骨密度高于LDN组小鼠（P＜0.05）；运动+LDN组小鼠股骨骨密度显著低于运动组小鼠（P＜0.01）。运动+LDN组小鼠血钙高于LDN组小鼠（P＜0.05）；同时， LDN组小鼠血钙显著高于对照组小鼠（P＜0.01），运动+LDN组小鼠血钙显著高于运动组小鼠（P＜0.01）。LDN组小鼠血磷高于对照组小鼠（P＜0.05），运动+LDN组小鼠血磷高于运动组小鼠（P＜0.05）。LDN组小鼠尿液脱氧吡啶啉排泄率高于对照组小鼠（P＜0.05），运动+LDN组小鼠尿液脱氧吡啶啉排泄率高于运动组小鼠（P＜0.05）。结论：运动可以促进生长期小鼠的骨密度，LDN--193189HCL的注射显著抑制了运动诱导的骨密度增加，BMP-Smad1信号转导途径介导了运动对骨吸收的作用，但对骨生物力学的作用却不明显。
关键词： 骨形态发生蛋白信号通路；运动；BMP-Smad1 阻滞剂；骨密度

ZHANG Lingli^1,， SUN Zhongguang^1,， LEI Le^2,， SHEN Chao^1,， ZOU Jun^3,*

（  1、Kinesiology School,Shanghai University of Sport,Shanghai 200438；       2、School of Economic and Management,Shanghai University of Sport,Shanghai 200438；       3、Development Planning Office，Shanghai University of Sport,Shanghai 200438；  ）

Abstract： Objective: The study tends to reveal how bone morphogenetic protein signaling pathways affects bone metabolism in the process of exercise training, via checking bone mineral density(BMD), biomechanics characteristics and blood and urine indices after injecting mice with Smad1 activating enzyme inhibitor, running a treadmill exercise or both. Methods: 72 six-week-old C57BL/6 male mice were divided into 4 groups as follows (18 mice for each group): control group, exercise group (medium intensity treadmill exercise), LDN group (injecting BMP-Smad1 inhibitor), exercise in combined with LDN group (both former interventions). After intervention, we check mineral density of femoral bone, biomechanics characteristics (maximum load, yield stress and elastic modulus), and blood and urine indices (serum calcium level, serum phosphate level, urinary DPD/Ucr). Results: Firstly, the BMD of exercise group was greatly higher than control group (P<0.01) and the BMD of exercise + LDN group was higher than LDN group (P<0.05), but it sharply lower than LDN group (P<0.01). The serum calcium level of exercise + LDN group was higher than LDN group (P<0.05). Moreover, the serum calcium level of LDN group was greatly higher than control group (P<0.01), and exercise + LDN group was greatly higher than exercise group (P<0.01). In addition, serum phosphate level of LDN group was higher than control group (P<0.05) and exercise + LDN group was higher than exercise group (P<0.05). DPD of LDN group was higher than control group (P<0.05) and exercise + LDN group was higher than exercise group (P<0.05). Conclusion: Exercise could improve BMD of growing mice, but injecting LDN-1931189HCL greatly inhibits the effect of exercise, which represents that BMP-Smad1 signaling pathway induces effects of exercise on bone resorption but it has no significant effect on bone biomechanics characteristics. Methods: 72 six-week-old C57BL/6 male mice were divided into 4 groups as follows (18 mice for each group): control group, exercise group (medium intensity treadmill exercise), LDN group (injecting BMP-Smad1 inhibitor), exercise in combined with LDN group (both former interventions). After intervention, we check mineral density of femoral bone, biomechanics characteristics (maximum load, yield stress and elastic modulus), and blood and urine indices (serum calcium level, serum phosphate level, urinary DPD/Ucr). Results: Firstly, the BMD of exercise group was greatly higher than control group (P<0.01) and the BMD of exercise + LDN group was higher than LDN group (P<0.05), but it sharply lower than LDN group (P<0.01). The serum calcium level of exercise + LDN group was higher than LDN group (P<0.05). Moreover, the serum calcium level of LDN group was greatly higher than control group (P<0.01), and exercise + LDN group was greatly higher than exercise group (P<0.01). In addition, serum phosphate level of LDN group was higher than control group (P<0.05) and exercise + LDN group was higher than exercise group (P<0.05). DPD of LDN group was higher than control group (P<0.05) and exercise + LDN group was higher than exercise group (P<0.05). Conclusion: Exercise could improve BMD of growing mice, but injecting LDN-1931189HCL greatly inhibits the effect of exercise, which represents that BMP-Smad1 signaling pathway induces effects of exercise on bone resorption but it has no significant effect on bone biomechanics characteristics.
Keywords： bone morphogenetic protein signaling pathways; exercise; BMP-Smad1 inhibitor; bone mineral density

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