张志伟， 周智超， 王泽根， 周田甜， 刘萌， 董宁征^*

（  苏州大学唐仲英血液学研究中心，苏州 215123；  ）

摘要： 目的 II型跨膜丝氨酸蛋白酶（type II transmembrane serine proteases，TTSPs）与非小细胞肺癌发生发展的关系。方法 利用基因表达数据库（gene expression omnibus，GEO）的全外显子基因芯片数据，计算癌组织与癌旁组织的基因相对表达水平，筛选差异表达基因；研究肺癌组织中TTSPs基因的相对表达量，筛查NSCLC 中特异性高表达的TTSPs，并分析其在不同期肺癌中的表达差异；检测肺癌细胞株中TTSPs mRNA的表达。结果 TMPRSS4、Matriptase、TMPRSS13在NSCLC中表达显著高于癌旁正常组织（P<0.001）；并且TMPRSS4的表达水平与肿瘤的分期密切相关（P<0.05）；肺癌细胞株中检测到TMPRSS4、Matriptase、TMPRSS13的表达。 结论 TTSPs中的TMPRSS4、Matriptase和TMPRSS13在非小细胞肺癌中有特异性高表达，可能与肿瘤的发生发展相关。????? 
关键词： II型跨膜丝氨酸蛋白酶；非小细胞肺癌；差异表达?????

ZHANG Zhiwei， ZHOU Zhichao， WANG Zhegen， ZHOU Tiantian， LIU Meng， DONG Ningzheng^*

（  Cyrus Tang Hematology Center, Soochow University, Suzhou 215123；  ）

Abstract： Objective The aim of the study is to investigate TTSPs expression in non-small cell lung cancer (NSCLC) samples. Methods The expression data of whole exon microarray were downloaded from gene expression omnibus (GEO), and analyzed using R language and packages of bioconductor. The differentially expression genes were determined by comparing caner tissues and adjacent normal tissues. TTSPs expression were further analyzed by TNM system. TTSPs mRNA expression in cell lines of lung cancer were analyzed by RT-PCR. Results TMPRSS4, Matriptase and TMPRSS13 expression levels were significantly up-regulated in NSCLC (P<0.0001 vs. normal tissues). TMPRSS4 expression levels seemed to correlate with the severity of disease. And TMPRSS4, Matriptase and TMPRSS13 mRNA expression were detected in the lung cancer cell lines by RT-PCR. Conclusions TMPRSS4, Matriptase and TMPRSS13 were abnormally up-regulated in NSCLC. Our results indicated that TTSPs may contribute to the pathogenesis of NSCLC. ?????
Keywords： Type II transmembrane serine protease; Non-small cell lung cancer; Differential expression

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