徐常新1,, 彭宣宪2,, 王三英1,, 李惠2,
( 1、厦门大学; 2、中山大学; )
职称论文发表摘要: 副溶血性弧菌(V. parahaemolyticus)具有嗜盐性,是引起海水养殖动物病害和海产品食物中毒的常见重要病原菌。本文采用月桂酰基氨酸钠二步法,提取了副溶血性弧菌的外膜蛋白,首次建立了外膜亚蛋白质组表达图谱,鉴定了29个外膜蛋白,占所鉴定蛋白的78.3%。这些研究结果对副溶血性弧菌外膜蛋白功能的研究具有积极作用。此外,对外膜蛋白组学研究的有关技术问题进行了相关探讨。
职称论文发表关键词: 副溶血性弧菌;外膜蛋白;蛋白质组学
Xu Changxin1,, Peng Xuanxian2,, Wang Sanying1,, Li Hui2,
( 1、Xiamen University; 2、Sun Yat-Sen University; )
Abstract: V. parahaemolyticus, a halophilic salt-requiring bacterium, is a important Gram-negative pathogen that cause great losses in aquaculture and sea food-borne diseases in human. In the present study, sub-outer membrane proteome of this bacterium was established using two-steps of sarcosine-insoluble fraction. Twenty-nine outer membrane proteins was identified, being 78.3% of the identified proteins. These results will be helpful in study of roles of V. parahaemolyticus outer membrane proteins. In addition, techniques on outer membrane proteome was addressed.
Keywords: V. parahaemolyticus;outer membrane protein;proteomics
中国科技论文在线:徐常新,彭宣宪,王三英等. 副溶血性弧菌外膜亚蛋白质组图谱的建立及其意义[EB/OL].北京:中国科技论文在线
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