董华， 樊超男， 付慧聪， 路媛媛， 齐可民*
（ 首都医科大学附属北京儿童医院、北京市儿科研究所营养研究室； ）
职称论文发表摘要： 探讨孕及哺乳期摄入n-6/n-3 PUFAs变化对子代脑功能相关基因表达的影响，使用清洁级C57BL/6J 3-4周龄雌性小鼠，随机分为5 组，分别给予n-3 PUFAs缺乏和4种不同含量n-3 PUFAs（n-6/n-3 PUFAs 比值分别为15:1、5:1、1:1及1:5）饲料喂养。小鼠12-14周龄时用常规饲料喂养的雄性小鼠与之合笼交配繁殖，仔鼠断乳后继续行母鼠相同饲料喂养，随访观察至生后7 d、21 d和3 m时分批处死后取脑。然后在仔鼠生后21d断乳时，随机挑选7-9只n-3 PUFAs缺乏组的仔鼠用n-6/n-3 PUFAs（5:1）组饲料喂养，至3m龄；同时随机挑选7-9只n-6/n-3 PUFAs（5:1）组的仔鼠，改用n-3缺乏组饲料喂养，至3m龄；剩余仔鼠用与母鼠相同的饲料喂养，至3m龄，麻醉牺牲后，取脑置于-80℃条件下备用。采用实时荧光定量PCR技术测定脑皮质gfap、mbp、nse、gad65、gad67、nr2b基因mRNA的表达。在各年龄段小鼠中，饲料中添加n-3 PUFAs使各基因的表达量明显增加；不同比例的n-6/n-3PUFAs对基因表达的影响不同。孕期及哺乳期饲料缺乏n-3PUFAs，即使断乳后的饲料中添加正常量的n-3 PUFAs，也不能使某些对脑功能起重要作用的基因的表达量升高至正常n-3 PUFAs喂养的小鼠。结果提示，孕期及哺乳期可能需要较高的n-3 PUFAs摄入（可能最优的n-6/n-3 PUFAs比例介于1:5~1:1之间），才能满足幼年期脑发育之需。保证生命早期n-3 PUFAs的适量摄入，有助于维持成年期脑功能相关基因的正常表达。
职称论文关键词： n-3多不饱和脂肪酸；脑发育相关基因；基因表达；早期营养；小鼠
DONG Hua， FAN Chaonan， FU Huicong， LU Yuanyuan， QI Kemin*
（ Nutrition Research Unit, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045； ）
Abstract： A mouse model was used in this study to explore the effects of maternal dietary n-6/n-3PUFAs during maternal pregnancy and lactation on the expressions of key brain genes in the offspring. Fifty 3-4 week-old C57BL/6J female mice were randomly divided into 5 groups, and were fed with five types of diets, the n-3 PUFA deficient diet, and four n-3 PUFAs diets with different ratios of n-6/n-3 PUFAs at 15:1, 5:1, 1:1 and 1:5 respectively. Followed by 8 weeks feeding, the mice were mated with male mice for breeding pups, which were fed with the same diets as their mothers after weaning. At postnatal 7, 21 d and 3 m respectively, pups were sacrificed and brains were used for detecting gene expression. To determine the effects of n-3 PUFAs status in early life on brain's gene expression in later life, equal male pups from groups of n-3 PUFAs deficient diet and n-6/n-3 PUFAs (5:1) diet were selected at postnatal 21 d, and were fed with the n-6/n-3 PUFAs (5:1) diet and the n-3 PUFA deficient diet respectively lasting for 2 months. At postnatal 7 d, 21 d or 3 m, compared to the n-3 PUFA deficient diet group, increased mRNA expressions of gfap, mbp, nse, gad65, gad67 and nr2b in the cerebral cortex were shown in the n-3 PUFA containing diet groups, and the effects were dependent on n-6/n-3 ratios. Compared to the mice with the n-3 PUFA deficiency during the whole life period in the experiment, the pups from the n-3 deficient diet group with feeding of the n-6/n-3 PUFAs (5:1) diet after weaning had similar mRNA expressions of gfap, mbp and nse, which were lower than those in mice with feeding of the n-6/n-3 PUFAs (5:1) diet during the whole experimental period. The data suggest that higher dietary intake of n-3 PUFAs during maternal pregnancy and lactation may be needed for better brain development and function, indicating that the optimal dietary ratio of n-6/n-3 PUFAs is likely between 1:5 to 1:1. N-3 PUFAs in early life may be benefitial for maintaining the transcription of brain genes in adults.
Keywords： n-3 polyunsaturated fatty acids; brain related genes; gene expression; early nutrition; mouse
作者简介： 董华（1987-），女，在读硕士研究生，主要研究方向为儿内科-营养
通信联系人： 齐可民（1967-），男，研究员/教授，主要研究方向为儿童营养
中国科技论文在线：董华，樊超男，付慧聪等. 孕期及哺乳期n-6/n-3脂肪酸变化对仔鼠脑发育相关基因表达的影响。
发表期刊：医学论文发表期刊
